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为研究田菁在盐胁迫下基因表达谱的变化，揭示其耐盐分子机理，采用RNA-Seq技术对NaCl胁迫处理不同时长的田菁根进行转录组测序，并进行de novo组装和功能注释，对差异表达基因进行生物信息学分析。结果表明：田菁在150 mmol·L-1 NaCl胁迫3和27 h时，分别产生571和1765个差异表达基因，这些差异表达基因功能主要涉及次生代谢、碳代谢、糖酵解及苯丙烷合成等过程；此外，分属于17和26个转录因子家族的32和66个转录因子表达水平在盐胁迫的不同阶段发生变化。综上，田菁对盐胁迫的响应是一个多基因参与、多个生物代谢过程协同调控的过程。本研究为揭示田菁耐盐分子机制及耐盐相关基因挖掘奠定了基础。
Abstract：To investigate the changes in gene expression profiles of Sesbania cannabina under salt stress and elucidate its molecular mechanisms of salt tolerance, the root transcriptomes of S. cannabina treated with NaCl for different durations were sequenced using RNA-Seq technology, followed by de novo assembly, functional annotation, and bioinformatic analysis of differentially expressed genes(DEGs).The results showed that 150 mmol·L-1 NaCl treatment for 3 h and 27 h induced 571 and 1,765 DEGs, respectively.These DEGs were primarily enriched in biological processes such as secondary metabolism, carbon metabolism, glycolysis, and phenylpropanoid biosynthesis.Additionally, 32 and 66 transcription factors(TFs),belonging to 17 and 26 TF families, exhibited altered expression levels at different stages of salt stress.In conclusion, the salt stress response in S.cannabina involves multiple genes and coordinated regulation of diverse biological metabolic processes.This study provides a foundation for deciphering the molecular mechanisms of salt tolerance and mining salt stress-related genes in S. cannabina.
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基本信息:
DOI：10.20062/j.cnki.CN37-1453/N.2025.04.007
中图分类号:S551.5;Q943.2
引用信息:
[1]王家祎,董萌,孔存翠,等.基于RNA-Seq技术的盐胁迫田菁转录组分析[J].鲁东大学学报(自然科学版),2025,41(04):343-351.DOI:10.20062/j.cnki.CN37-1453/N.2025.04.007.
基金信息:
国家自然科学基金(32071733,32371915); 山东省自然科学基金(ZR2023QC034);山东省自然科学基金(ZR2024MH247); 山东省烟台市高校与地方企业合作项目(2021XDRHXMPT09); 山东省现代农业产业技术体系创新团队项目(SDAIT-02-05)