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外套膜是长牡蛎贝壳形成的重要器官，外套膜三个褶皱中也存在色素沉着，并与牡蛎壳色相关。本研究通过对黑壳、白壳长牡蛎外套膜三褶皱的组织学观察和转录组分析，在黑色外套膜外褶皱中筛查到多个可能与长牡蛎贝壳形成和贝壳色素沉着相关的基因：几丁质相关基因、酪氨酸酶Tyr基因、酸性基质蛋白Pif基因。这些基因主要在外褶皱高表达，可能通过调控几丁质和色素的合成、结合、代谢降解等过程，参与调控贝壳形成和贝壳色素沉着。此外，在黑色内、中褶皱显著富集到purple和lightcyan两个模块，两个模块的关键基因Bdh2和TRIM45可能在能量代谢及免疫调控中发挥作用。本研究通过对长牡蛎外套膜三层褶皱转录组数据的系统分析，进一步解析长牡蛎壳色形成机制，为长牡蛎的壳色育种奠定分子基础。
Abstract：Mantle is an important organ for shell formation in oyster.There is also pigment deposition in the three mantle folds, which are related to the shell color in Crassostrea gigas.This study screened genes which may play key roles in shell formation and shell pigmentation in oyster by histological observation and transcriptome analysis of black-and white-three mantle folds.Chitin related genes, tyrosinase(Tyr) genes, and acid matrix protein(Pif) genes were mainly highly expressed in black outer fold and may participate in regulating shell formation and shell pigmentation by regulating processes such as chitin and pigment synthesis, binding, and metabolic degradation.In addition, the black inner-and middle-folds have significantly enriched two modules by WGCNA.Two hub genes Bdh2 and TRIM45 in the purple and lightcyan module may play roles in energy metabolism and immune regulation.This study systematically analyzed the transcriptome data of the three mantle folds in C.gigas.It will further elucidate the mechanism of shell pigmentation and will lay a molecular foundation for shell color breeding in oyster.
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基本信息:
DOI：10.20062/j.cnki.CN37-1453/N.2025.04.001
中图分类号:S917.4
引用信息:
[1]袁孟强,刘坤,杨李俊淞,等.基于外套膜三褶皱转录组解析长牡蛎贝壳色素沉着机制[J].鲁东大学学报(自然科学版),2025,41(04):289-297.DOI:10.20062/j.cnki.CN37-1453/N.2025.04.001.
基金信息:
国家自然科学基金(42076088); 山东省重点研发计划(农业良种工程)(2022LZGC015); 山东省泰山学者专项资金(tstp20240518); 山东省现代农业产业技术体系(SDAIT-14-01); 烟台市省级以上领军人才专项配套经费(202203); 烟台市水产种业提升项目(2023HD)