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脱落酸(Abscisic acid, ABA)在植物响应干旱胁迫中发挥着重要作用，其中，9-顺式环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase, NCED)是ABA生物合成的关键限速酶。本研究在小麦全基因组范围内鉴定出13个NCED基因家族成员，并对其系统进化关系、基因结构、保守结构域及在不同组织和干旱胁迫下的表达模式进行了分析。结果表明，TaNCED基因的进化关系越近，它们的内含子-外显子结构特征和保守结构域越相似。转录组数据表明，不同TaNCED基因对干旱胁迫的响应模式各异。其中，TaNCED2-A、TaNCED3-B/D和TaNCED4-A/B/D在干旱胁迫后表达显著上调，且TaNCED4-A/B/D在干旱胁迫的表达量最高；TaNCED1-A/D在所有检测时间点均未表达；TaNCED5-A随着干旱胁迫时间的延长，其表达量逐渐降低。本研究为深入解析小麦NCED基因家族在干旱胁迫响应中的作用机制提供了理论基础，也为小麦抗旱分子育种提供了潜在的基因靶点。
Abstract：Abscisic acid(ABA) plays a crucial role in plant responses to drought stress, and 9-cis-epoxycarotenoid dioxygenase(NCED) is a key rate-limiting enzyme in ABA biosynthesis. In this study, a total of 13 NCED genes were identified in the wheat genome, and their phylogenetic relationships, gene structures, conserved domains, and expression patterns in different tissues and under drought stress were analyzed. The results showed that TaNCED genes with closer evolutionary relationship had the more similar their intron-exon structural features and conserved domains. Transcriptome data revealed that different TaNCED genes exhibited diverse response patterns to drought stress. Specifically, the expression of TaNCED2-A,TaNCED3-B/D,and TaNCED4-A/B/D was significantly up-regulated after drought stress treatment, and TaNCED4-A/B/D showed the highest expression level under drought stress; TaNCED1-A/D was not expressed at all detected time points; and the expression of TaNCED5-A gradually decreased with the prolongation of drought stress. This study provides a theoretical basis for deeply understanding the mechanism of the wheat NCED gene family in response to drought stress and offers potential gene targets for drought-resistant molecular breeding in wheat.
[1] AMOAH J N,SEO Y W.Effect of progressive drought stress on physio-biochemical responses and gene expression patterns in wheat[J].3 Biotech,2021,11(10):440.
[2] YANG G,PAN Y,PAN W Q,et al.Combined GWAS and eGWAS reveals the genetic basis underlying drought tolerance in emmer wheat (Triticum turgidum L.)[J].New Phytologist,2024,242(5):2115-2131.
[3] ZHANG H M,ZHU J H,GONG Z Z,et al.Abiotic stress responses in plants[J].Nature Reviews Genetics,2021,23(2):104-119.
[4] HSU P K,DUBEAUX G,TAKAHASHI Y,et al.Signaling mechanisms in abscisic acid-mediated stomatal closure[J].The Plant Journal,2021,105(2):307-321.
[5] 王慧，汲宁宁，钱关泽.小麦脱落酸(ABA)信号传导途径研究进展[J].聊城大学学报(自然科学版),2022,35(5):94-102.
[6] CHEN K,LI G J,BRESSAN R A,et al.Abscisic acid dynamics,signaling,and functions in plants[J].Journal of Integrative Plant Biology,2020,62(1):25-54.
[7] HWANG S G,LEE C Y,TSENG C S.Heterologous expression of rice 9-Cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance[J].Botanical Studies,2018,59(1):2.
[8] IUCHI S,KOBAYASHI M,TAJI T,et al.Regulation of drought tolerance by gene manipulation of 9-Cis-epoxycarotenoid dioxygenase,a key enzyme in abscisic acid biosynthesis in Arabidopsis[J].The Plant Journal,2001,27(4):325-333.
[9] 李嘉伟，徐佳灿，崔建华，等.苹果NCED基因家族鉴定及表达分析[J].山东农业大学学报(自然科学版),2025,56(2):285-292.
[10] TAN B C,JOSEPH L M,DENG W T,et al.Molecular characterization of the Arabidopsis 9-Cis epoxycarotenoid dioxygenase gene family[J].The Plant Journal,2003,35(1):44-56.
[11] 李康，聂小军，方桂英，等.普通小麦及其近缘种NCED基因的克隆及表达分析[J].西北农业学报，2010,19(6):55-59.
[12] CHEN Y M,SONG W J,XIE X M,et al.A collinearity-incorporating homology inference strategy for connecting emerging assemblies in the triticeae tribe as a pilot practice in the plant pangenomic era[J].Molecular Plant,2020,13(12):1694-1708.
[13] CHEN C J,WU Y,LI J W,et al.TBtools-II:a “one for all,all for one” bioinformatics platform for biological big-data mining[J].Molecular Plant,2023,16(11):1733-1742.
[14] BAILEY T L,BODEN M,BUSKE F A,et al.MEME SUITE:tools for motif discovery and searching[J].Nucleic Acids Research,2009,37(Web Server issue):W202-W208.
[15] LESCOT M,DéHAIS P,THIJS G,et al.PlantCARE,a database of plant Cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequencesOpen Access[J].Nucleic Acids Research,2002,30(1):325-327.
[16] VON MERING C,HUYNEN M,JAEGGI D,et al.STRING:a database of predicted functional associations between proteins[J].Nucleic Acids Research,2003,31(1):258-261.
[17] HEYN P,KALINKA A T,TOMANCAK P,et al.Introns and gene expression:cellular constraints,transcriptional regulation,and evolutionary consequences[J].BioEssays,2015,37(2):148-154.
[18] LEE S U,MUN B G,BAE E K,et al.Drought stress-mediated transcriptome profile reveals NCED as a key player modulating drought tolerance in Populus davidiana[J].Frontiers in Plant Science,2021,12:755539.
[19] TOLNAI Z,SHARMA H,SOóS V.D27-like carotenoid isomerases:at the crossroads of strigolactone and abscisic acid biosynthesis[J].Journal of Experimental Botany,2024,75(4):1148-1158.
基本信息:
DOI：10.20062/j.cnki.CN37-1453/N.2025.04.006
中图分类号:S512.1
引用信息:
[1]刘浩,马于翌,孙欣,等.小麦NCED基因家族鉴定及其在干旱胁迫下的表达模式分析[J].鲁东大学学报(自然科学版),2025,41(04):335-342.DOI:10.20062/j.cnki.CN37-1453/N.2025.04.006.
基金信息:
山东省重点研发计划(2024LZGCQY012,2022LZG002-2); 泰山学者计划(20230119); 烟台市科技计划项目(2023ZDCX023)