| 200 | 0 | 30 |
| 下载次数 | 被引频次 | 阅读次数 |
为探究蟹类对滨海盐沼湿地土壤的生物扰动效应，本研究利用16S rRNA高通量测序技术，分析黄河三角洲盐沼湿地蟹洞土壤的细菌群落结构和功能及其关联的土壤因子。结果发现：(1)蟹洞土壤的含水率、电导率、总有机碳和碳氮比等理化性质均有显著变化，且变形菌门、拟杆菌门和放线菌门等优势菌群也存在显著差异；(2)蟹洞土壤的细菌群落多样性与丰富度均显著低于对照土壤，其细菌群落结构与对照土壤存在显著差异；(3)蟹洞土壤细菌群落表现出更高的网络连通性和模块化，加强了细菌个体间的网络联系；(4)新陈代谢是土壤细菌群落的主要代谢功能，蟹洞土壤细菌群落的三级代谢功能与对照土壤存在显著差异；(5)细菌群落α多样性与土壤电导率、总有机碳和含水率等环境因子显著相关，土壤电导率和总有机碳显著影响了土壤优势菌群和群落功能。本研究为深入揭示底栖蟹类对滨海盐沼湿地土壤的生物掘洞效应提供了一定的理论参考。
Abstract：In order to explore the bioturbation effect of crabs on the soil of coastal salt marsh wetland, 16S rRNA high-throughput sequencing technology was used to analyze the bacterial community structure and function of crab burrows and the associated soil factors in the salt marsh wetland of the Yellow River Delta.The results are shown as follows.(1) The soil moisture content, electrical conductivity, total organic carbon, carbon nitrogen ratio and other physicochemical properties of the crab burrow soil are significantly changed, and the dominant bacteria groups such as Proteobacteria, Bacteroideta and Actinomyces are also significantly different.(2) The diversity and richness of bacterial community in the crab burrow soil are significantly lower than that in the control soil, and the bacterial community structure is significantly different from that in the control soil.(3) The bacterial communities in crab burrow soil show higher network connectivity and modularity, which strengthens the network connections among bacterial individuals.(4) Metabolism is the main metabolic function of soil bacterial community, and the tertiary metabolic function of bacterial community in the crab burrow soil is significantly different from that in the control soil.(5) The α diversity of bacterial community is significantly correlated with environmental factors such as soil conductivity, total organic carbon and water content, and soil conductivity and total organic carbon significantly affects soil dominant flora and community function.This study provides a theoretical reference for further revealing the biological burrowing effect of benthic crabs on coastal salt marsh wetland soil.
[1] 骆梦，王青，邱冬冬，等.黄河三角洲典型潮沟系统水文连通特征及其生态效应[J].北京师范大学学报(自然科学版),2018,54(1):17-24.
[2] 崔丽娟，李伟，窦志国，等.近30年中国滨海滩涂湿地变化及其驱动力[J].生态学报，2022,42(18):7297-7307.
[3] 艾熙航，辛琨，曾书军.滨海湿地潮沟演变及其生态效应研究进展[J].湿地科学，2024,22(4):603-616.
[4] 王金庆.长江口盐沼优势蟹类的生境选择与生态系统工程师效应[D].上海：复旦大学，2008.
[5] 陈雪，贺强，辛沛，等.河口海岸潮滩蟹类生物扰动行为过程研究进展[J].海洋科学，2021,45(10):113-122.
[6] XIAO K,WU Y C,PAN F,et al.Widespread crab burrows enhance greenhouse gas emissions from coastal blue carbon ecosystems[J].Communications Earth & Environment,2024,5(1):437.
[7] GROW A K,SCHUTTE C A,ROBERTS B J.Fiddler crab burrowing increases salt marsh greenhouse gas emissions[J].Biogeochemistry,2022,158(1):73-90.
[8] GUIMOND J A,SEYFFERTH A L,MOFFETT K B,et al.A physical-biogeochemical mechanism for negative feedback between marsh crabs and carbon storage[J].Environmental Research Letters,2020,15(3):034024.
[9] 解雪峰，项琦，吴涛，等.滨海湿地生态系统土壤微生物及其影响因素研究综述[J].生态学报，2021,41(1):1-12.
[10] AN J X,LIU C,WANG Q,et al.Soil bacterial community structure in Chinese wetlands[J].Geoderma,2019,337:290-299.
[11] WU C J,WU H T,LIU D D,et al.Crab bioturbation significantly alters sediment microbial composition and function in an intertidal marsh[J].Estuarine,Coastal and Shelf Science,2021,249:107116.
[12] QIU D D,CUI B S,YAN J G,et al.Effect of burrowing crabs on retention and accumulation of soil carbon and nitrogen in an intertidal salt marsh[J].Journal of Sea Research,2019,154:101808.
[13] 陈友媛，刘道彬，贾永刚，等.生物活动对黄河口潮滩表层沉积物扰动作用的研究[J].中国海洋大学学报(自然科学版),2007,37(5):829-833.
[14] WANG X H,LI Y Z,GUAN B,et al.Beneficial effects of crab burrowing on the surface soil properties of newly formed mudflats in the Yellow River Delta[J].Ecohydrology & Hydrobiology,2020,20(4):548-555.
[15] FANJUL E,ESCAPA M,MONTEMAYOR D,et al.Effect of crab bioturbation on organic matter processing in South West Atlantic intertidal sediments[J].Journal of Sea Research,2015,95:206-216.
[16] LI J L,HUA G F,LIU S Q,et al.Effects of crab disturbance on nitrogen migration and transformation in a coastal tidal flat wetland[J].Environmental Science and Pollution Research,2021,28(37):52345-52356.
[17] 罗康宁，廖安邦，肖孟阳，等.滩涂湿地蟹类活动对表层土壤的扰动效应[J].生态科学，2021,40(6):83-88.
[18] 王维奇，徐玲琳，曾从盛，等.河口湿地植物活体—枯落物—土壤的碳氮磷生态化学计量特征[J].生态学报，2011,31(23):134-139.
[19] 陈桂香，陈淑云，王维奇，等.闽江河口湿地螃蟹对枯落物分解的影响[J].亚热带资源与环境学报，2017,12(3):35-42.
[20] 解璐萌，查玟琦，车纯广，等.黄河三角洲盐沼湿地螃蟹洞穴形态结构对土壤水盐运移过程的影响[J].生态与农村环境学报，2024,40(6):831-839.
[21] WU X F,YANG J J,RUAN H,et al.The diversity and co-occurrence network of soil bacterial and fungal communities and their implications for a new indicator of grassland degradation[J].Ecological Indicators,2021,129:107989.
[22] ZHANG Z S,WEI Z,WANG J J,et al.Ants alter molecular characteristics of soil organic carbon determined by pyrolysis-chromatography/mass spectrometry[J].Applied Soil Ecology,2018,130:91-97.
[23] CHEN W D,WEN D H.Archaeal and bacterial communities assembly and co-occurrence networks in subtropical mangrove sediments under Spartina alterniflora invasion[J].Environmental Microbiome,2021,16(1):10.
[24] MA B,WANG Y L,YE S D,et al.Earth microbial co-occurrence network reveals interconnection pattern across microbiomes[J].Microbiome,2020,8(1):1-12.
[25] ZILIUS M,BONAGLIA S,BROMAN E,et al.N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont[J].Scientific Reports,2020,10(1):13966.
[26] 侯建伟，邢存芳，杨莉琳，等.生物炭与有机肥等碳量投入土壤肥力与细菌群落结构差异及关系[J].环境科学，2024,45(7):4218-4227.
[27] QIN G M,LU Z,GAN S C,et al.Fiddler crab bioturbation stimulates methane emissions in mangroves:insights into microbial mechanisms[J].Soil Biology and Biochemistry,2024,194:109445.
[28] AN Z R,GAO D Z,CHEN F Y,et al.Crab bioturbation alters nitrogen cycling and promotes nitrous oxide emission in intertidal wetlands:influence and microbial mechanism[J].Science of the Total Environment,2021,797:149176.
[29] SIMIONI F,CAMPOS V A,DORADO-RODRIGUES T F,et al.Crab burrows and termite thermal chimneys as refuges for anurans in a neotropical wetland[J].Salamandra,2014,50(3):133-138.
[30] 孙刚，房岩，毕语涵.底栖动物对沉积物稳定性的扰动效应[J].农业与技术，2014,34(9):163-183.
[31] 康小虎，刘鑫，刘姗姗，等.不同时空尺度下土壤微生物群落分布及驱动因素研究进展[J].土壤，2024,56(4):689-696.
[32] RATH K M,MAHESHWARI A,ROUSK J.Linking microbial community structure to trait distributions and functions using salinity as an environmental filter[J].mBio,2019,10(4):e01607-19.
[33] KAMBLE P N,GAIKWAD V B,KUCHEKAR S R,et al.Microbial growth,biomass,community structure and nutrient limitation in high pH and salinity soils from Pravaranagar(India)[J].European Journal of Soil Biology,2014,65:87-95.
[34] ADAMCZYK M,PEREZ-MON C,GUNZ S,et al.Strong shifts in microbial community structure are associated with increased litter input rather than temperature in High Arctic soils[J].Soil Biology and Biochemistry,2020,151:108054.
[35] BASTIDA F,ELDRIDGE D J,GARCíA C,et al.Soil microbial diversity-biomass relationships are driven by soil carbon content across global biomes[J].The ISME Journal,2021,15(7):2081-2091.
[36] AGUSTO L E,QIN G M,THIBODEAU B,et al.Fiddling with the blue carbon:fiddler crab burrows enhance CO2 and CH4 efflux in saltmarsh[J].Ecological Indicators,2022,144:109538.
[37] 史祯琦，佘冬立，陈心逸，等.盐分对土壤氨挥发与反硝化速率的影响[J].生态与农村环境学报，2021,37(11):1458-1464.
基本信息:
DOI：10.20062/j.cnki.CN37-1453/N.2025.04.002
中图分类号:X171;S154.3
引用信息:
[1]王永琪,闫军芬,王志康,等.蟹类掘洞对滨海盐沼湿地土壤微生物群落结构及功能的影响[J].鲁东大学学报(自然科学版),2025,41(04):298-307.DOI:10.20062/j.cnki.CN37-1453/N.2025.04.002.
基金信息:
国家自然科学基金—山东联合基金(U2106214); 国家自然科学基金(42271055); 山东省自然科学基金(ZR2023MD088); 山东省高等学校青创团队(2022KJ118)